Premaxillary Advancement by Using Distraction Osteogenesis and Evaluation the Effects of TGFβ1 on Bone Formation


  • Dilay Şahan
  • Müfide Dinçer
  • Gökhan Alpaslan
  • Cansu Alpaslan

Received Date: 01.06.2010 Accepted Date: 19.08.2010 Turk J Orthod 2010;23(3):212-225


The aim of this experimental study was to evaluate the advancement of premaxilla with a bone-tooth borne distractors using the landmarks on standard lateral cephalograms and the effects of TGFβ1 on bone healing.

Materials and Methods:

Fourteen rabbits were divided in 3 groups where first group (n=7) was treated with distraction osteogenesis, second group (n=5) was treated with distraction osteogenesis combined with external TGFβ1 application, and 2 rabbits were treated only with corticotomy as controls. Following anesthesia and adaptation of the individual distractors, corticotomy of palatal bone and lateral maxillary walls for distraction was made. The distraction has started 3 days following the surgical procedure, and the device was activated 0.6 mm each day for 8 days. External TGFβ1 application was made following 24 hours of the initial expansion in animals where distraction was combined with the application of growth factor. Animals were sacrificed after 12 days of retention period and specimens were collected for histological examination.


The amount of anterior movement of the premaxilla was found 3.79 mm for the distraction osteogenesis group and 3.7 mm for the group where external TGFβ1 application was made following distraction. ConcCleft palate, alveolar distraction, secondary alveolar bone, histological bone healing was much better in TGFβ1 treated group.

Keywords: Distraction osteogenesis, premaxillary advancement, bone-tooth borne distraction appliance, TGFβ1