Turkish Journal of Orthodontics
Original Article
Contamination of Low Frictional Elastomeric Ligatures by Streptococcus mutans: A Prospective RT-PCR and AFM Study

Contamination of Low Frictional Elastomeric Ligatures by Streptococcus mutans: A Prospective RT-PCR and AFM Study

1.

Department of Orthodontics, Dentistry Faculty, Gaziantep University, Gaziantep, Turkey

2.

Department of Microbiology, Medical Faculty, Gaziantep University, Gaziantep, Turkey

3.

Department of Chemistry, Art and Science Faculty, Gaziantep University, Gaziantep, Turkey

Turk J Orthod 2021; 34: 163-169
DOI: 10.5152/TurkJOrthod.2021.20091
Read: 148 Downloads: 67 Published: 20 September 2021

Objective: To compare Streptococcus mutans colonization between low-friction elastomeric ligatures and to correlate microbial colonization
levels with the surface roughness status.

Methods: The study included 160 premolars of 10 patients. During the study period, which consisted of 4 sessions each lasting 4 weeks, the ligature types Slide™ Low-Friction Ligature (Leone, Firenze, Italy), Tough-O Energy™ (Rocky Mountain Orthodontics, Denver, USA), and Sili Ties™ (Dentsply Sirona, Surrey KT13 0NY, UK), and steel ligatures (American Orthodontics, Sheboygan, USA) as a control, were fixed to the premolar teeth by clockwise rotation among the jaw quadrants. The plaque index (PI) and gingival index (GI) were obtained before bonding (T0), 6 weeks after bonding (T1), and subsequently every 4 weeks (T2, T3, T4). Presence of S. mutans was analyzed by real-time polymerase chain reaction at T1, T2, T3, T4. Surface roughness was evaluated with Atomic Force Microscopy (AFM) before ligation (Ra0) and after (Ra1) ligation. The paired t-test, ANOVA, repeated measures of ANOVA, and the Kruskal–Wallis test were used for the statistical analysis.

Results: S. mutans colonization was significantly higher on the Slide group (P < .05). The lowest Ra0 was seen in Slide and the highest was seen in the Tough-O Energy group. There was no correlation between S. mutans colonization and Ra1 parameters of elastomeric groups (P > .05).

Conclusion: S. mutans colonization showed variations in low-friction elastomeric ligatures independent of surface roughness. Ringshaped low-friction elastomeric ligatures were not different from the steel ligature in terms of S. mutans colonization.

Cite this article as: Dagdeviren C, Gulec A, Eksi F, Saglam M, Kahraman M. Contamination of low frictional elastomeric ligatures by Streptococcus mutans: A prospective RT-PCR and AFM study. Turk J Orthod. 2021; 34(3): 163-169.

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